abstract：:The effects of phenylalanine (PHE) and its deaminated metabolites phenylpyruvate (PHP), phenyllactate (PHL) and phenylacetate (PHA) on sodium and potassium activated adenosinetriphosphatase (Na+,K+-ATPase) in synaptosomes from rat brain were investigated. At very low concentrations (5-10 microM). PHE, PHL and PHA inhi...

journal_title：Neurochemical research

authors： Dwivedy AK,Shah SN

更新日期：1982-06-01 00:00:00

abstract：:Mitochondrially bound brain hexokinase is solubilized by anesthetics and this effect has been suggested to contribute to anesthesia. In the present investigation the influence of the metabolic inhibitor 2-deoxy-D-glucose (2-DOG) was studied. An isolated rat brain preparation was used to avoid the contribution of perip...

journal_title：Neurochemical research

authors： Krieglstein J,Sperling G,Stock R

更新日期：1982-06-01 00:00:00

abstract：:Post-ischemic changes in energy metabolites and natural antioxidant compounds have been measured in rat brain in vitro concurrent with two different assays for peroxidized lipids. No exogenous free radical initiators were employed. In vitro oxygenation of minced brain preparations for periods of 10 minutes to 4 hours,...

journal_title：Neurochemical research

authors： Kogure K,Watson BD,Busto R,Abe K

更新日期：1982-04-01 00:00:00

abstract：:Alpha adrenergic receptor subtypes in rat hippocampal membranes were studied, using [3H] clonidine as the radioactive ligand. On the basis of competitive binding studies, using the selective antagonist-prazosin, WB-4101, and yohimbine, [3H] clonidine appeared to bind to a population of presynaptic sites that are pharm...

journal_title：Neurochemical research

authors： George CR

更新日期：1982-02-01 00:00:00

abstract：:A simple, rapid method for determining depolarization-induced 45Ca influx into synaptosomes is described. Synaptosomes which had been depolarized in the presence of 45Ca were applied to a small column of Chelex-100 resin to separate free Ca2+ from that taken up by the tissue. Approximately 70% of the synaptosomal prot...

journal_title：Neurochemical research

authors： Michaelis ML,Michaelis EK,Simpson AE

更新日期：1982-02-01 00:00:00

abstract：:Abnormal myo-[2-3H]inositol incorporation into phosphatidylinositol has been found in phentolamine-treated synaptosomes that were isolated from the cerebral hemispheres of galactose toxic rats and incubated with [33P]Pi and myo-[2-3H] inositol. In galactose toxic rats phentolamine-stimulated myo-[2-3H]inositol labelin...

journal_title：Neurochemical research

authors： Berry G,Yandrasitz JR,Segal S

更新日期：1982-01-01 00:00:00

abstract：:Cell densities and respiratory rates of astrocytes from neonatal rat brain grown in primary culture were determined after 20-30 days in vitro. Cells grown in flasks reached lower densities (microgram DNA/cm2) and higher protein:DNA ratios than cells grown in petri dishes. Respiratory rates were lower for cells grown i...

journal_title：Neurochemical research

authors： Olson JE,Holtzman D

更新日期：1981-12-01 00:00:00

abstract：:Metabolism of triacylglycerol (TAG) in developing brain has been examined. TAG is a relatively minor fraction of brain lipid in both suckling and adult rats and cannot be accounted for as entrapped blood. When glycerol tri[1-14C]oleate and [2-3H]glycerol trioleate were simultaneously injected intracerebrally into suck...

journal_title：Neurochemical research

authors： Cook HW

更新日期：1981-11-01 00:00:00

abstract：:Rabbits were immunized with the myelin-associated glycoprotein (MAG) that had been purified from isolated rat brain myelin by selective extraction with lithium diiodosalicylate (LIS) and phenol followed by preparative SDS gel electrophoresis. Antibodies to MAG were detected qualitatively by immunodiffusion and quantit...

journal_title：Neurochemical research

authors： Quarles RH,Johnson D,Brady RO,Sternberger NH

更新日期：1981-10-01 00:00:00

abstract：:Turnover rate of individual molecular species of sphingomyelin of adult rat brain myelin and microsomal membranes was determined after an intracerebral injection of 100 microCi of [C3H3]choline. Myelin and microsomal membrane sphingomyelins were isolated from the rest of the lipids. The individual molecular species of...

journal_title：Neurochemical research

authors： LeBaron FN,Sanyal S,Jungalwala FB

更新日期：1981-10-01 00:00:00

abstract：:Rat obturator nerve 16S acetylcholinesterase (16S AChE) was separated by sucrose gradient velocity sedimentation and compared to the 16S form of AChE similarly derived from endplate regions of anterior gracilis muscles. The 16S AChE from both tissues could only be extracted in high ionic strength buffer; as it aggrega...

journal_title：Neurochemical research

authors： Fernandez HL

更新日期：1981-09-01 00:00:00

abstract：:Mevalonate phosphorylation was studied in neonatal chick brain. Formation of phosphorylated derivatives of mevalonic acid increased with the pH in the range assays (5.5-9.5). Phosphomevalonate kinase was completely inactivated after treatment at 50 degree C for 5-10 min, whereas mevalonate kinase was found to retain i...

journal_title：Neurochemical research

authors： Linares A,Aguilera JA,García-Peregrín E

更新日期：1981-08-01 00:00:00

abstract：:The effects of 1 min of acute hypoxic treatment (1% O2 in N2) on incorporation of [1-14C]arachidonic acid into brain lipids of 16-day-old rats were investigated at 3, 6, and 12 min after intracerebral injection of the labeled fatty acid. The hypoxic-hypoxia condition associated with convulsive seizures caused a decrea...

journal_title：Neurochemical research

authors： Strosznajder J,Sun GY

更新日期：1981-07-01 00:00:00

abstract：:Quantitative measurements were made of choline acetyltransferase (CAT) activity, acetylcholinesterase (AChE) activity and cholinergic muscarinic receptor binding ([3H]QNB) in eight areas of a cross-section of the rat medulla oblongata. A fourth cholinergic parameter, high-affinity choline uptake, was measured in three...

journal_title：Neurochemical research

authors： Simon JR,Oderfeld-Nowak B,Felten DL,Aprison MH

更新日期：1981-05-01 00:00:00

abstract：:Microvessels were isolated from a bovine cortex and the transport of glucose was investigated by using 2-deoxy-D-[3H]glucose (2-DG). The apparent Km for 2-DG transport was 118 microM and therefore indicates a significant high affinity for the substrate. The inhibition of 2-DG uptake by D-glucose showed an apparent Ki ...

journal_title：Neurochemical research

authors： Stefanovich V,Gojowczyk G

更新日期：1981-04-01 00:00:00

abstract：:Disruptive effects of calcium upon neurofilaments and glial filaments were studied in white matter of rat optic nerve and spinal cord and in rat peripheral nerve. Filament ultrastructure and tissue protein composition were compared following a calcium influx into excised tissues. A calcium influx was induced by freeze...

journal_title：Neurochemical research

authors： Schlaepfer WW,Zimmerman UP

更新日期：1981-03-01 00:00:00

abstract：:Rabbits sensitized with whole nervous tissue or myelin basic protein (MBP) plus adjuvant and developing experimental allergic encephalomyelitis (EAE) were studied for the presence of oligoclonal immunoglobulin (Ig) bands in spinal fluid and serum. Samples obtained prior to sensitization and at the time of sacrifice we...

journal_title：Neurochemical research

authors： Whitacre CC,Mattson DH,Paterson PY,Roos RP,Peterson DJ,Arnason BG

更新日期：1981-01-01 00:00:00

abstract：:Evidence is presented in support of the working hypothesis that "prenervous" neurotransmitters directly participate in cell-cell interactions occurring during the first several cleavage divisions of sea urchin embryos, a function which may occur during the early development of higher animals as well. This intercellula...

journal_title：Neurochemical research

authors： Buznikov GA,Shmukler YB

更新日期：1981-01-01 00:00:00

abstract：:Isozyme patterns of cholinesterase (ChE) from heart, tongue, and skeletal muscle of normal and dystrophic hamsters are presented. Two principal bands, bands 1 and 2, were evaluated. Band 1 migrates faster towards the anode than does band 2. While bands 1 and 2 stain for AChE and were found in control muscles, only ban...

journal_title：Neurochemical research

authors： Henderson NS,Tweedle CD,Kabara JJ

更新日期：1980-12-01 00:00:00

abstract：:The changes in both the levels of some free amino acids and their metabolism in the rat brain during the first 24 hr of postnatal life were studied. The content of glutamic acid decreased for the first 2 hr; it remained at the lowest level for the next 4 hr, when it began to increase. The content of alanine decreased ...

journal_title：Neurochemical research

authors： Noguchi T,Nomura M,Tsukada Y

更新日期：1980-10-01 00:00:00

abstract：:The effect of isoproterenol, norepinephrine, dopamine, and serotonin on the accumulation of adenosine 3',5'-monophosphate (cAMP) was studied in homogeneous neuronal cultures from 8-day chick embryo hemispheres. Among the catecholamines, isoproterenol had a more pronounced effect on the accumulation of cAMP. Norepineph...

journal_title：Neurochemical research

authors： Ciesielski-Treska J,Ulrich G

更新日期：1980-10-01 00:00:00

abstract：:The acetylcholinesterase from synaptosomal membranes is inhibited by anesthetics: Nembutal, brietal, and thiopental. Nembutal and brietal decrease the Km for acetylthiocholine, without changes in Vmas. A noncompetitive type of inhibition is produced by thiopental. This anesthetic decreases Arrhenius plot discontinuity...

journal_title：Neurochemical research

authors： Pastuszko A

更新日期：1980-07-01 00:00:00

abstract：:The K-stimulated release of [3H]ACh from rat midbrain slices prelabeled by incubation with [3H]choline was dependent on extracellular Ca. Phenobarbital inhibited the K-stimulated [3H]ACh release and the IC50 was equal to that found for K-stimulated endogenous ACh release. These results support the suggestion that barb...

journal_title：Neurochemical research

authors： Richter JA,Jackson SK

更新日期：1980-07-01 00:00:00

abstract：:We describe an attempt to incorporate a metabolically inert phospholipid analog into animal membranes, especially myelin, in vivo, with the view of eventual long-term membrane modification or membrane engineering. A sonicated suspension of a mixture of [14C]phosphatidylcholine and its dialkyl analog, [3H] tetradecylo...

journal_title：Neurochemical research

authors： Brockerhoff H,Wisniewski HM,Lipton LC,Deshmukh DS

更新日期：1980-06-01 00:00:00

abstract：:Metabolic turnover of axonally transported glycoproteins has been examined in membranous and soluble subfractions of goldfish optic tectum following intraocular injection of [3H]fucose. Radioactivity in total transported glycoproteins reached a maximum in the tectum after 24-30 hr, then declined with a half-life of ap...

journal_title：Neurochemical research

authors： Ripellino JA,Elam JS

更新日期：1980-04-01 00:00:00

abstract：:Poly(A)-RNA prepared from the brains of 30-day-old male rats has been shown to direct the synthesis of neuron-specific enolase (NSE) in a cell-free system derived from rabbit reticulocytes. The newly synthesized polypeptides were immunoprecipitated and analyzed on SDS-polyacrylamide slab gels. Autoradiography indicate...

journal_title：Neurochemical research

authors： Walker WA,Zomzely-Neurath C

更新日期：1980-04-01 00:00:00

abstract：:One pathway by which tryptophan is metabolized in the brain as well as in the periphery is through cleavage of the indole ring to formylkynurenine and then kynurenine. Indoleamine-2,3-dioxygenase, the enzyme that catalyzes this cleavage, and kynurenine are distributed all across the different anatomic regions of brain...

journal_title：Neurochemical research

authors： Gál EM,Sherman AD

更新日期：1980-03-01 00:00:00

abstract：:Mice infected with the Venezuelan equine encephalomyelitis virus showed a significant decrease in the GABA content of cerebral hemispheres. Activity of the enzyme which synthetizes GABA, glutamate decarboxylase, is also reduced in whole cerebral hemispheres, neostriatum, and frontal cortex of infected animals, as comp...

journal_title：Neurochemical research

authors： Bonilla E,Ryder E,Ryder S

更新日期：1980-02-01 00:00:00

abstract：:Three peptides overlapping the tryptophan region of bovine CNS myelin basic protein were synthesized by the solid phase procedure of Merrifield. These were the nonapeptide H-Phe-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH, the octapeptide H-Ser-Trp-Gly-Ala-Glu-Gly-Gln-Lys-OH, and the heptapeptide H-Trp-Gly-Ala-Glu-Gly-Gln-Lys-...

journal_title：Neurochemical research

authors： Levit S,Powers JM,Milek D,Brostoff SW

更新日期：1980-01-01 00:00:00

abstract：:The kinetics of the uptake of p-tyramine, m-tyramine, and dopamine were investigated in slices of the hypothalamus and striatum of the rat in the presence of nialamide. When uptake was analyzed by a least-squares fit to a Lineweaver-Burk plot, each amine appeared to be concentrated by both a "low"-affinity and a "high...

journal_title：Neurochemical research

authors： Petrali EH,Boulton AA,Dyck LE

更新日期：1979-10-01 00:00:00

abstract：:The activities of L-glutamate decarboxylase (GAD), GABA-transaminase (GABA-T), choline acetyltransferase (CAT), and cysteic and cysteinesulfinic acids decarboxylase (CAD/CSAD) in putamen and frontal cortex in both Huntington's chorea and normal tissues were measured. The greatest difference between Huntington's and no...

journal_title：Neurochemical research

authors： Wu JY,Bird ED,Chen MS,Huang WM

更新日期：1979-10-01 00:00:00

abstract：:The influence of theophylline (2.5--100 mg/kg p.o.) on cyclic 3',5'-adenosine monophosphate (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) in brain of Sprague-Dawley rats (0.5--3.0 hr after administration of theophylline) was investigated. It was found that theophylline increases cAMP and cGMP levels when admi...

journal_title：Neurochemical research

authors： Stefanovich V

更新日期：1979-10-01 00:00:00

abstract：:In a study of the role of taurine in the genesis of epilepsy the effects of metrazol-induced convulsions on the uptake and distribution of taurine in the brain were measured. In vivo we found no significant uptake of taurine in the mouse brain; in rabbit brain in most areas significant taurine uptake was found. The ph...

journal_title：Neurochemical research

authors： Battistin L,Varotto M,Tezzon F,Pistollato L

更新日期：1979-08-01 00:00:00

abstract：:Rat tissues were tested for their ability to inhibit the binding of [3H]dihydromorphine or [3H]naloxone to membrane-bound opiate receptors. By this criterion, morphine-like substances were found in lung, heart, liver, and kidney as well as in brain. The relative activity of the extracts, based on initial tissue weight...

journal_title：Neurochemical research

authors： Neidle A,Manigault I,Wajda IJ

更新日期：1979-06-01 00:00:00

abstract：:A sensitive and rapid assay method method for cysteic acid decarboxylase was develped which combined the selectivity of ion exchange resin (a complete retention of the substrate, cysteic acid, and exclusion of the product, taurine) with the speed of a vacuum filtration. The synthesis and purification of 35S-labeled cy...

journal_title：Neurochemical research

authors： Wu JY,Moss LG,Chen MS

更新日期：1979-04-01 00:00:00

abstract：:The effect of postdecapitative ischemia (5 min at 37 degrees) and hypoxia (5% O2, 95% N2, 30 min) on the distribution of protein radioactivity in the cellular fractions of guinea pig cerebral cortex was studied. Ischemic conditions resulted in the increase of total radioactivity level and protein content in the mitoch...

journal_title：Neurochemical research

authors： Rossowska M,Zalewska T

更新日期：1979-02-01 00:00:00

abstract：:Rats fed a vitamin E-deficient diet for 7--8 weeks postweaning showed no change in brain weight or the activity in brain of various enzymes involved in neurotransmitter synthesis and metabolism. Body and muscle weights were markedly reduced. Muscle choline acetyltransferase and acetylcholinesterase activities were sig...

journal_title：Neurochemical research

authors： Sung SC,Sandberg PR,McGeer EG

更新日期：1978-12-01 00:00:00

abstract：:The axonal transport of tRNA has been investigated in the chick optic system. Chicks were injected with [3H]uridine intraocularly or intracranially and the RNA of the retina, nerve complex, and tecta separated by polyacrylamide gel electrophoresis and then counted. The ratio of tRNA to rRNA specific activities increas...

journal_title：Neurochemical research

authors： Por S,Gunning PW,Jeffrey PL,Austin L

更新日期：1978-08-01 00:00:00

abstract：:Hypothalamic and plasma dopamine-beta-hydroxylase [DBH, EC 1.14.2.1] activites were measured by a coupled radioenzymatic method. Animals representing five experimental groups [intact controls, adrenalectomized, pinealectomized, adrenalectomized pinealectomized, doubly sham-operated] were killed and sampled at 8 times ...

journal_title：Neurochemical research

authors： Banerji TK,Quay WB,Kachi T

更新日期：1978-06-01 00:00:00

abstract：:A rapid and specific method for assaying 4-aminobutyrate-2-oxoglutarate aminotransferase was developed. The method was based on the selectivity of ion exchange resin and the speed of vacuum filtration. With this new method, the aminotransferase activity in various tissues has been determined as follows: brain, 10.2; s...

journal_title：Neurochemical research

authors： Wu JY,Moss LG,Chude O

更新日期：1978-04-01 00:00:00